OAP Projects in the GULF OF ALASKA


Next-Gen gene sequencing to understand effects of ocean acidification on Alaskan crab and fish

Chris Long - NOAA/NMFS Alaska Fisheries Science Center

Thursday, May 26, 2022

Using next-generation sequencing techniques to assess adaptive capacity and illuminate mechanisms underlying the effects of high pCO2 on Alaskan crab and fish species

Why we care
Many economically important crab and fish species are negatively affected by exposure to ocean acidification predicted to occur throughout their ranges in the coming decades. Ocean acidification results in decreased growth, altered development, weaker exoskeletons, increased energy outputs, altered immune systems, altered behavior, and increased mortality in some of these species. Other stressors such as increased temperature can have interactive negative effects when combined with ocean acidification. Traditional laboratory experiments cannot duplicate the gradual changes that will affect species populations over multiple life-history stages and generations, so using next-generation genetic approaches provide insight into effects beyond specific life stages.

What we are doing 
This study will use next-generation sequencing techniques to identify specific alterations in the molecular, metabolic, and physiological pathways of individuals exposed to ocean acidification. This is a way to identify pathways that impart tolerance to ocean acidification and warming. This project determines the effect of oceanacidification and thermal stress on gene expression in Pacific cod larvae and juvenile Tanner crab and identifies genetic markers indicating ocean acidification resilience. 

Benefits of our work
Investigators will identify the cellular pathways that impart tolerance to ocean acidification. By comparing individuals that demonstrate low sensitivity to ocean acidification and with the general population, we enhance the ability to predict how adaptation will alter the species’ response to future ocean conditions. This research will inform the fishing industry and coastal, fisheries-dependent Alaskan communities about potential effects of ocean change on commercially important species. Outcomes can be used to drive future responses and adaptations in these industries regarding affected fisheries.

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